Matrix Metalloproteinase-1, Human Recombinant, HEK 228-12251 from RayBiotech | Biocompare.com

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Matrix Metalloproteinase-1, Human Recombinant, HEK

Product Specs
ItemMatrix Metalloproteinase-1, Human Recombinant, HEK
CompanyRayBiotech
Price Pricing Info Supplier Page View Company Product Page
Catalog Number228-12251
Quantity10 ug, 1 mg
NCBI Full Gene Namematrix metallopeptidase 1
NCBI Gene AliasesCLGN, CLG
PurityGreater than 95% as determined by SDS-PAGE
TypeEnzymes
Source/Expression SystemHEK 293 cells
FormatSterile Filtered colorless solution
ActivityThe activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-KPLGL-Dpa-AR-NH2, The specific activity is >, 400 pmoles/min/ ug.Recombinant Human MMP-1 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA).Activation Protocol:1. Dilute MMP1 to 50 ug/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.2. Activate MMP1 by adding APMA to a final concentration of 1mM. (Sigma, Catalog # A9563) and 100mM stock in DMSO.3. Incubate at 37°,C for 2 hours

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RayBiotech

RayBiotech

3607 Parkway Lane, Ste. 200

Norcross, Georgia 30092

United States

Phone: (888) 494-8555
(770) 206-2393

Company Profile

Website: http://www.raybiotech.com

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